TABLE 3.
Heat-induced RpoH synthesis is inhibited by rifampina
| Time after heat shock (min) | Rifampin (200 μg/ml) | Relative rate of RpoH synthesis |
|---|---|---|
| 0 | − | 1 |
| 3 | − | 3.2 |
| 5 | − | 3.8 |
| 3 | + | 0.6 |
| 5 | + | 0.4 |
a
Strain GV3100 was grown in synthetic medium at 25°C to mid-log phase and shifted to 37°C with (+) or without (−) rifampin added 1 min before temperature upshift. Samples were taken at the times indicated and labeled with [35S]methionine for 2 min, and immunoprecipitates were analyzed by SDS-PAGE to determine the rate of RpoH synthesis as described in the legend to Fig. 1B. The synthesis rates are presented relative to the value at zero time.