Figure 6. Combining a HFD and osmotic mini pump‐infused STZ model is mediated by a dose‐dependent reduction in pancreatic beta cell mass.

A, pancreatic sections were immunolabelled for pan‐insulin (green; labels proinsulin and insulin) and counterstained with DAPI to visualise nuclei, allowing identification of islets of Langerhans (dashed line). B, number of islets per mm² of pancreas across the treatment groups. C, average islet area. D, percent of islet area with positive pan‐insulin immunoreactivity. Data are the mean ± SD; each point represents mean data from an individual animal. Scale bar = 50 μm. One‐way ANOVA with Student–Newman–Keuls post hoc was used to assess differences between groups. P values are main effects of treatment vs. the control group. [Colour figure can be viewed at wileyonlinelibrary.com]