FIGURE 1.

Study design. Naked, LNP1 and LNP2‐packaged hGALT mRNAs provided by Moderna Inc. were injected in CG zebrafish (galt KO) zebrafish at one‐cell stage (0–1 h post fertilization, hpf) and intravenous (48–56 hpf). GALT activity, protein expression (merely one‐cell stage injections) and Gal‐1‐P and galactonate (merely intravenous injections) were assessed at 5 days post fertilization (dpf). LNP, lipid nanoparticle; mRNA, messenger RNA; hpf, hours post fertilization; dpf, days post fertilization; LNP1, LNP1‐packaged mRNA; LNP2, LNP2‐packaged mRNA; Naked, naked mRNA; Gal‐1‐P, galactose‐1‐phosphate. Figure is created with BioRender.com