FIGURE 5.

Effects Bacillus subtilis (BS) feeding on the proportion of peripheral CD3⁺ T cells during lactation. During the present lactation, the control group (n = 10) was fed a normal diet, and the experimental group (n = 10) was fed a BS diet between 1 month before parturition and the end of the lactation. Peripheral blood was obtained from the dairy cows on 12 occasions from just before parturition to the end of lactation. The percentages of CD4⁺ T cells, CD8⁺ T cells, γδ⁺ T cells, WC1⁺ γδ⁺ T cells and CD8⁺ γδ⁺ T cells were analyzed in CD3⁺ T cells by flow cytometry (a). The comparison analysis shows the differences in CD3⁺ T cells between the control group and the BS‐feeding group in the total lactation period, the early and the late stages of lactation, respectively (b). *P < 0.05, **P < 0.01.