TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Markersa and derivation | Source or reference(s) |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH10B | recA1 endA1 | GIBCO/BRL |
| S17-1 | recA endA thi hsdR RP4-2 Tc::Mu::Tn7 Tpr Smr | 34 |
| S. meliloti | ||
| RU11/001 | Smr spontaneous streptomycin-resistant wild-type strain | 29 |
| RU11/011 | Smr Nmr ΔflaA ΔflaB ΔflaC ΔflaD | 29, 35 |
| RU11/820 | Smr ΔflaA | This work |
| RU11/821 | Smr ΔflaB | This work |
| RU11/822 | Smr ΔflaC | This work |
| RU11/823 | Smr ΔflaD | This work |
| RU11/824 | Smr ΔflaB ΔflaC | This work |
| RU11/825 | Smr ΔflaB ΔflaD | This work |
| RU11/826 | Smr ΔflaC ΔflaD | This work |
| RU11/833 | Smr ΔflaA ΔflaB | This work |
| RU11/834 | Smr ΔflaA ΔflaC | This work |
| RU11/835 | Smr ΔflaA ΔflaB ΔflaD | This work |
| RU11/836 | Smr ΔflaA ΔflaC ΔflaD | This work |
| RU11/837 | Smr ΔflaB ΔflaC ΔflaD | This work |
| RU11/842 | Smr ΔflaA ΔflaB ΔflaC | This work |
| R. lupini | ||
| RU12/001 | Smr, spontaneous streptomycin-resistant wild-type strain | This work |
| RU12/002 | Smr ΔflaA | This work |
| RU12/003 | Smr ΔflaB | This work |
| RU12/004 | Smr ΔflaD | This work |
| RU12/005 | Smr ΔflaB ΔflaD | This work |
| RU12/006 | Smr ΔflaA ΔflaB ΔflaD | This work |
| RU12/007 | Smr ΔflaA ΔflaD | This work |
| Plasmids | ||
| pK18mobsacB | KmrblacZ mob sacB | 30 |
| pPHU234c | Tcr, promoterless lacZ fusion | 14 |
| pUCBM20 | Apr | Boehringer Mannheim |
| pRU738 | Apr Tcr (tet) | 40 |
| pRU1993 | Kmrb Tcr, recombinant of a 3,028-bp EcoRI/PstI tet gene from pRU738 and pK18mobsacB | 25 |
| pRU2274 | Tcr, recombinant of a 545-bp EcoRI/PstI fragment containing the flaA promoter of S. meliloti and pPHU236 | 35 |
| pRU2275 | Tcr, recombinant of a 263-bp flaB EcoRI/PstI fragment of the flaB promoter of S. meliloti and pPHU236 | 25 |
| pRU2276 | Tcr, recombinant of a 397-bp flaD XhoI/PstI fragment of the flaD promoter of S. meliloti and pPHU234 | 25 |
| pRU2277 | Tcr, recombinant of a 311-bp flaC EcoRI/PstI fragment of the flaC promoter of S. meliloti and pPHU235 | 25 |
| pRU2351 | Kmr Tcr, recombinant of a 10.7-kb genomic MluI fragment containing flaA and flaB of R. lupini RU12/001 and pRU1993 | This work |
| pRU2352 | Kmr Tcr, recombinant of a 8.9-kb genomic HindIII/PstI fragment containing flaD of R. lupini RU12/001 and pRU1993 | This work |