TABLE 2.

Properties of R. lupini and S. meliloti flagellin mutants

Organism	Relevant genotype	Effective Fla subunit(s)	Swarm size (%)a	Vinst (μm/s)b	Filament morphologyc
R. lupini	Wild type	ABD	100	37.3 ± 3.4	Normal
	ΔflaA	BD	0	0	Straight, severely truncated (Fig. 4B)
	ΔflaB	AD	93 ± 3	36.1 ± 3.2	Normal
	ΔflaD	AB	74 ± 6	35.1 ± 2.9	Normal
	ΔflaAD	B	0	0	Straight, severely truncated
	ΔflaBD	A	0	0	Straight, (numbers low)
	ΔflaABD		0	0	No flagella
S. meliloti	Wild type	ABCD	100	38.6 ± 3.1	Normal
	ΔflaA	BCD	6 ± 2	—e	Enhanced curvature, severely truncated (Fig. 4E)
	ΔflaB	ACD	19 ± 1	26.6 ± 2.4	Normal (reduced numbers)
	ΔflaC	ABD	39 ± 4	30.7 ± 2.0	Normal (reduced numbers)
	ΔflaD	ABC	62 ± 2	36.2 ± 3.3	Normal, fragile
	ΔflaAB	CD	0	0	NDd
	ΔflaAC	BD	0	0	ND
	ΔflaBC	AD	7 ± 3	20.5 ± 1.8	Normal (reduced numbers)
	ΔflaBD	AC	21 ± 2	25.7 ± 1.6	ND
	ΔflaCD	AB	26 ± 2	26.3 ± 2.1	ND
	ΔflaABD	C	0	0	No flagella
	ΔflaACD	B	0	0	No flagella
	ΔflaBCD	A	0	0	Rare extensions, no fine structure
	ΔflaABCD		0	0	No flagella

^aPercentage of wild-type swarm diameter (after deduction of 6-mm diameter of inoculum) on 0.3% Bromfield agar after 36 h at 30°C. Values are means of results from four replicates. 

^bInstantaneous velocity (absolute speed) averaged for every swimming track. Mean values for 1,000 individual tracks were determined from each sample by computerized motion analysis and were averaged from at least five independent cell populations. 

^cAppearance on electron micrographs. 

^dND, not determined. 

^e—, the movement was jiggly.