Figure 1.

Schematic overview of the number of systemic sclerosis (SSc) patients included in ELISA 1, Luminex and ELISA 2. (A) In the previously published ELISA 1, 297 SSc patients and 100 random healthy controls were included. Of these 167 patients had undergone by RHC and 41 patients were diagnosed with PAH. In the Luminex analysis, serum samples from 458 SSc patients form Oslo University Hospital (OUH), 93 serum samples form SSc patients from Zurich University Hospital (USZ) and the random healthy controls were run, as well as 68 age and gender matched healthy controls. In total, 251/456 patients included in the Luminex analysis had undergone RHC and 72 were diagnosed with PAH. For the follow-up ELISA 2, 176 serum samples form OUS, 49 serum samples from USZ and 60 age and gender matched healthy controls were included. Of these patients, 152 had conducted RHC and 51 were diagnosed with PAH. (B) The samples overlapping in the three different runs were 243 samples between ELISA1 and Luminex, 80 in ELISA1 and ELISA2, and 218 in Luminex and ELISA 2. The number of samples overlapping in all three runs were 76 samples.