FIG. 6.

Cellular localization of the S16-RimM hybrid protein. A polysome extract of strain JML020 (rimM120 rpsP877) was fractionated by sucrose gradient centrifugation, and the indicated fractions were screened for the presence of the S16-RimM hybrid protein by using an anti-RimM antiserum in a Western blotting experiment. The locations of the 50S and 30S ribosomal subunits, 70S ribosomes, and polysomes are indicated below the A₂₆₀ curve. The identity of native-sized RimM protein was determined by running a total extract of the wild-type strain MW100 and a molecular weight marker on the protein gel.