FIGURE 2.

cAMP activation and inhibition profiles of N‐terminally truncated GLP‐2 variants on the human GLP‐2R. COS‐7 cells were transiently transfected with human GLP‐2R and assessed for either cAMP activation or inhibition upon stimulation with the GLP‐2 truncated variants of (a) GLP‐2(2‐33), (b) GLP‐2(3‐33), (c) GLP‐2(4‐33), (d) GLP‐2(5‐33), (e) GLP‐2(6‐33), (f) GLP‐2(7‐33), (g) GLP‐2(8‐33), (h) GLP‐2(9‐33), (i) GLP‐2(10‐33) or (j) GLP‐2(11‐33). The dashed line in each graph represents human GLP‐2. Data are shown as mean ± SEM, from n = 3 independent experiments carried out in duplicate