FIGURE 3.

cAMP activation and inhibition profiles of GLP‐2(3‐33)[D3A] on the human GLP‐2R. (a) Schematic overview of GLP‐2(3‐33)[D3A]. The black dot indicates where the amino acid has been substituted. COS‐7 cells were transiently transfected with the human GLP‐2R and assessed for (b) competition with [¹²⁵I]‐GLP‐2(1‐33)[M10Y] or (c) cAMP activation or inhibition with GLP‐2(3‐33)[D3A]. The dashed line represents human GLP‐2. Data are shown as mean ± SEM, from n = 3 independent experiments carried out in duplicate