FIGURE 7.

Selectivity test of the N‐terminally truncated and lipidated GLP‐2 variants on the human GLP‐1R. COS‐7 cells were transiently transfected with the human GLP‐2R or human GLP‐1R and assessed for either cAMP activation (a) or inhibition (b) with GLP‐2(3‐33)(C16‐diacid/3), GLP‐2(4‐33)(C16‐diacid/4) and GLP‐2(5‐33)(C16‐diacid/5) or cAMP activation (c) or inhibition (d) with GLP‐2(3‐33)[N16K](C16‐diacid/16) and GLP‐2(3‐33)[R20K](C16‐diacid/20). The full lines represent the human GLP‐1R, and the dashed lines the human GLP‐2R. Data are shown as mean ± SEM, from n = 3 independent experiments carried out in duplicate