TABLE 1.
Purification of OleY methyltransferase activity
Purification step | Sp act (pmol · min−1 · mg of protein−1) | Purification (fold) |
---|---|---|
Cell extraction | 0.03 | 1 |
(NH4)2SO4 precipitation | 0.04 | 1.52 |
Phenyl-Sepharose elution | 0.05 | 1.52 |
Sephacryl S-200 elution | 0.99 | 29.77 |
Q-Sepharose elution | 2.56 | 76.89 |