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. 2001 Sep;183(18):5358–5363. doi: 10.1128/JB.183.18.5358-5363.2001

TABLE 1.

Purification of OleY methyltransferase activity

Purification step Sp act (pmol · min−1 · mg of protein−1) Purification (fold)
Cell extraction 0.03 1
(NH4)2SO4 precipitation 0.04 1.52
Phenyl-Sepharose elution 0.05 1.52
Sephacryl S-200 elution 0.99 29.77
Q-Sepharose elution 2.56 76.89