FIGURE 3.

Recommended NTRK gene fusion testing algorithm for adult sarcomas, adapted from expert recommendations from the World Sarcoma Network. ⁴⁴ (+)/(–): Presence/absence of NTRK gene fusions. †Please note that this is the opinion of the expert panel. Confirmation of NTRK gene fusions is required for TRK inhibitors. ^‡For confirmation test, NGS is preferred, but FISH or RT‐PCR may be used if NGS is not accessible. Abbreviations: ALK, anaplastic lymphoma kinase; BRAF, v‐raf murine sarcoma viral oncogene homolog B; CDK4, cyclin‐dependent kinase 4; GIST, gastrointestinal stromal tumor; IHC, immunohistochemistry; KIT, v‐kit Hardy‐Zuckerman 4 feline sarcoma viral oncogene homolog; LPS, liposarcoma; MDM2, mouse double minute 2 homolog; NF1, neurofibromatosis type 1; NGS, next‐generation sequencing; NTRK, neurotrophic tyrosine receptor kinase; PDGFRA, platelet‐derived growth factor receptor alpha; ROS1, ROS proto‐oncogene 1, receptor tyrosine kinase; SDH, succinate dehydrogenase. ^*Specific histologies to look out for include, but are not limited to, lipofibromatosis‐like neural tumours, peripheral nerve sheath tumours, female reproductive organ sarcomas, undifferentiated sarcomas, osteosarcoma