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Isolation of proHlyA and HlyA. Lysates of uninduced (−) and induced (+) cell cultures of E. coli BL21(DE3)(pT7HlyA) and the resulting purified proHlyA protein (2 μg loaded). Secreted HlyA (2 μg) purified from the culture supernatant (snt) of E. coli MC1061 (pEK50). Samples were analyzed by SDS-10% PAGE and Coomassie staining and size-calibrated using standard molecular weight markers.
