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. 2022 Aug 29;36(10):e22529. doi: 10.1096/fj.202200522R

FIGURE 1.

FIGURE 1

SIRT6 regulates both SMAD3‐dependent and ‐independent pathways in HSCs. (A) Immunoblot and quantification analysis of SIRT6 and SMAD3 in LX‐2 cells transfected with sgGFP, sgSMAD3, sgSIRT6, or sgSMAD3+sgSIRT6 CRISPR/Cas9 plasmids in the presence of 5 ng/ml of TGF‐β1 for 24 h (n = 3). Actinin (ACTN) was used as a loading control. (B) Real‐time PCR analysis of COL1A1, COL3A1, CTGF, and CYR61 in the LX‐2 cells transfected with sgGFP, sgSMAD3, sgSIRT6, or sgSMAD3+sgSIRT6 in the absence (−) or presence of 5 ng/ml of TGF‐β1 for 24 h (n = 4). Data are presented as mean ± SEM. *p < .05, **p < .01, and ***p < .001, and ### p < .001 for sgSMAD3+sgSIRT6 versus sgSMAD3.