FIGURE 4.

The enhanced clonogenicity of SmoA1/Nbn ^HZ granule cell progenitors (GCPs) depends on Notch signalling. (A) Microfluidic card qPCR analysis of the indicated transcripts in SmoA1/Nbn ^WT and SmoA1/Nbn ^HZ GCP neurospheres. mRNA expression levels were normalised on the mean expression of four reference genes (Pgk1, Hprt, Gusb, and Tfrc), and relative mRNA quantification was expressed as fold change. Data are reported as mean (±SD) of five replicates. p‐Values were calculated by two‐sided Student's t‐test. Statistically significant differences (p < 0.05) were observed for Hes1 and Hes5 (marked with a red asterisk). (B) Neurosphere formation assay from P7 SmoA1/Nbn ^WT and SmoA1/Nbn ^HZ GCPs. Neurospheres were dissociated and plated for the analysis in the presence of DMSO, DAPT or GDC‐0449. Data are expressed as the ratio of drug‐ versus DMSO‐treated samples. p‐Values were calculated by two‐sided Student's t‐test (**p < 0.01; ns, not significant). (C) qPCR analysis of the indicated transcripts in GCP neurospheres treated as in B