FIGURE 6.

Nbn depletion impairs Sonic Hedgehog (SHH)‐medulloblastoma (MB) growth in vitro and in vivo. (A) Western blot (WB) analysis of healthy wild‐type (WT) cerebella at P21 (C) and MBs explanted from mice with the indicated genotypes. Blots were probed with the indicated antibodies, and β‐actin was used as a loading control. Data are representative of at least three replicates. (B) WB analysis of MB‐neurospheres subjected to Nbn RNAi (Nbni) or no target RNAi (NoTi) for 5 days. (C) WB analysis of MB‐neurospheres treated with mirin or vehicle. Data are representative of three replicates. (D) evaluation of tumour growth in MB‐neurosphere allografts; data are expressed as mean volume ± SEM (n = 5 per group). Day 1 represents the first day in which tumour mass reached a measurable size. p‐Values were calculated by two‐sided Student's t‐test (*p < 0.05). (E) Kaplan–Meier survival curve of MB‐neurosphere allografts. Mice were sacrificed when tumours reached a volume of ≥2 cm³ or alternatively at Day 66. MS, median survival. p‐Values were calculated by a Gehan–Breslow–Wilcoxon test (*p < 0.05).