FIG. 1.

Growth and RpoS levels in the presence of relA multicopy in P. aeruginosa. (A) Growth curves of PAO1(pMMB67) (closed squares), PAO-R1(pMMB67) (open squares), PAO1(pMRL4) (diamonds), and PAO-R1(pMRL4) (circles) in LB medium. (B) Cellular RpoS levels during the growth of PAO1(pMMB67). Cells were grown in LB medium, and samples were taken at the turbidity at 600 nm (OD₆₀₀) indicated above each lane. Immunoblot analysis was performed with 50 μg of total cellular protein applied per lane. (C) Same experiment as in panel B with the PAO1(pMRL4) strain. (D) PAO-R1(pMMB67) strain. (E) PAO-R1(pMRL4) strain.