. 2001 Sep;183(18):5376–5384. doi: 10.1128/JB.183.18.5376-5384.2001

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristics^a	Source or reference
E. coli strains
TG1	supE Δ(lac-proAB) thi hsdRΔ5 (F′ traD36 proA⁺B⁺lacI^q ZΔM15)	29
E. coli [λI₁4]	Δ(argF-lac) λ::lasIp-lacZ	41
P. aeruginosa strains
PAO1	Wild type, prototroph	B. W. Holloway
PAO-R1	PAO1 ΔlasR::tet Sm^r	15
PAO-JP2	ΔlasI ΔrhlI Tc^r Hg^r	35
PAΩR3	PAO1 relA::pKoRΔ3	This work
SS24	PAO1 rpoS101::aacCI Gm^r	45
Plasmids
pMMB67	IncQ tacp lacI^q Ap/Cb^r	14
pLAFR3	pLAFR1 derivative, IncP1 oriT cos Tc^r	11
pSM10	E. coli relA⁺	40
pMRL4	pMMB67 with E. coli relA under tacp	This work
pLFR2	pLAFR3 with P. aeruginosa relA gene	This work
pMAL.S	IncP Tc^rP. aeruginosa rpoS′-lacZ fusion	26
pMAL.R	IncP Tc^rlasR′-lacZ fusion	26
pMAL.V	IncP Tc^rrhlR′-lacZ fusion	26
pRK2013	ColE1 Tra⁺ Mob⁺ (pRK2) Km^r	10
pECP61.5	ColE1 ori_{P. aeruginosa} rhlAp-lacZ fusion tacp-rhlR⁺ Ap^r	35
pPCS1	ColE1 lasR⁺ Ap^r	41
pKoRΔ3	pUC19 with an 0.48-kb internal fragment of P. aeruginosa relA Ap/Cb^r	This work

Ap^r, ampicillin resistant; Cb^r, carbenicillin resistant; Gm^r, gentamicin resistant; Hg^r, mercury resistant; Km^r, kanamycin resistant, Sm^r, streptomycin resistant; Tc^r, tetracycline resistant.