Figure 6.

AIEC infection alters host epigenome in HF-fed mice, enhancing AIEC colonization. a: Western blot targeting HDAC1, HDAC5 and H3ac expression in colonic mucosa of Chow-fed mice uninfected or infected with AIEC strain LF82. G: HDAC1, HDAC5 and H3ac accumulation in colonic mucosa of HF-fed mice uninfected or infected with AIEC strain LF82 analyzed by western blot. b-e, h-k: Quantification of protein expression was performed by assessing band intensities using the Image Lab software (n = 7 mice/per group). Bars represent medians. Mann-Whitney test, **p < .01. f, l: Correlation between AIEC LF82 load associated with the colonic mucosa of Chow-fed mice (f) or HF-fed mice (l) and global H3 acetylation level. Correlation existing between two variables was assessed by a Spearman test. H3ac: acetylated H3.