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. 2022 Oct 5;14(1):2127172. doi: 10.1080/19420862.2022.2127172

Figure 1.

Size exclusion chromatogram for unstressed mAb1 as well as mAb1incubated for 1 month at 37°C and 50°C. Elevated aggregate levels are observed after temperature stressing, with the highest levels in the 50°C condition. B) Size exclusion chromatogram for purified dimers isolated from unstressed mAb1 and the stressed samples described in A). In each sample, the dimer is the predominant species. C) SDS PAGE for unstressed mAb1 and the three dimer samples. Non-reduced analysis on left hand side showing presence of SDS-resistant dimer in all three dimer samples. Reduced analysis on the right-hand side showing that the majority of SDS-resistant dimer is reducible.

Generation and isolation of dimers from mAb1. a) SEC chromatograms for unstressed mAb1, and mAb1 after incubation for 1 month at 37°C and 50°C. b) SEC chromatograms of isolated dimers. c) SDS-PAGE analysis of the dimer samples. SM = starting material (mAb1), ND = Native dimers, 37 = 37°C dimers, 50 = 50°C dimers. Samples were analyzed non-reduced and reduced. Monomer is shown in green boxes, SDS-resistant dimer is shown in white boxes. Under reduced conditions, LC and HC are shown in blue and red boxes, respectively, with non-reducible species shown in purple boxes.