Skip to main content
. 2022 Oct 5;14(1):2127172. doi: 10.1080/19420862.2022.2127172

Figure 6.

Summary of PCA ELISA and HDX-MS results. A) Homology model of mAb1 showing regions implicated in dimer interface identified using PCA ELISA and HDX-MS for the 37°C dimer. Implicated regions are found in the Fab and Fc domains. B) Homology model of mAb1 showing regions implicated in dimer interface identified using PCA ELISA and HDX-MS for the 50°C dimer. Fewer regions were implicated compared to the 37°C dimer.

Summary of PCA ELISA and HDX data visualized using a homology model of mAb1. a) Results for HDX-MS and PCA ELISA for the 37C dimer sample. Residues which showed protection in the PCA ELISA are colored Orange, for the HDX protected residues are colored yellow. Residues which showed protection in both techniques are shown in purple. All other residues shown in cyan. b) Results for HDX-MS and PCA ELISA for the 50C dimer sample. Colors are the same as for A).