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. 2022 May 29;52(7):1171–1189. doi: 10.1002/eji.202149480

Figure 2.

Figure 2

Flow cytometry analysis of circulating follicular helper T (cTfh) cells and their subsets in peripheral blood samples from common variable immunodeficiency and autoimmunity (CVID) patients with respect to controls (HC). (A) Representative flow cytometry plots for Tfh (CXCR5+CD4+), follicular Treg (FoxP3+CXCR5−), and conventional Treg (CXCR5−CD4+), gated on singlets lymphocytes, CD3+CD14−CD8−CD19−. Percentages of cTfh (B), cTfr (C), cTfh:cTfr ratio (D), and cTfh subsets in peripheral blood of CVID patients compared to age‐matched HC. From left to right: (E) frequencies of cTfh1 subset (CXCR3+ CCR6−), (F) cTfh17 (CXCR3− CCR6+), (G) cTfh2 (CXCR3− CCR6−). Data are pooled from more than 10 independent experiments (CVID n = 27, HD n = 106). In all graphs, dots represent individual donors and asterisks indicate statistical significance as calculated by Mann–Whitney test. Black bars: median with interquartile range. *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001.