Fig. 2.

SFXN3 is imported similar to the AAC import pathway in a ΔΨ and ATP dependent manner. (A) ³⁵S‐labelled AAC or SFXN3 were imported into wild‐type yeast mitochondria in the presence or absence of ΔΨ and ATP before being subject to SDS‐PAGE or BN‐PAGE. (B) ³⁵S‐labelled AAC or SFXN3 were arrested in stage III of the carrier import pathway (ΔΨ−) for 10 min followed by re‐isolation and import in the presence of membrane potential (ΔΨ+) for the indicated timepoints. Samples were then subject to SDS‐PAGE or BN‐PAGE. Porin was probed as a housekeeping gene at 29 kDa. 5% = 5% of protein loaded into all other lanes. Each stage indicates a point in the carrier import process [29, 55, 61, 62].