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Relative incorporation of 4fW by an evolved and ancestral clone. GST was purified from clones B7-3 and C600p grown on 95% 4fW medium, as described above. HPLC-ESI MS was performed. Traces for the masses of 1,094 and 1,112 Da are shown. (A) A peptide isolated from C600p shows a roughly equal proportion of 1,094- and 1,112-Da peptides at about 6.6 and 7 min. (B) HPLC-ESI MS of a peptide produced by B7-3 shows an absence of any significant peak at ∼6.6 min of a mass of 1,094 Da.
