TABLE 1.
Primers used for amplification and sequencing of genesa
Gene | Primerb | Primer sequence |
---|---|---|
aroP | 22.APA | 5′-GACGATTTACGGGGCGTTGGTG-3′ |
24.APB | 5′-CATTCTACATATTGAGAGGGGTTG-3′ | |
22.AP-iA1 | 5′-CGCCAGGCTAATCATCGCCCAG-3′ | |
20.AP-iA2 | 5′-CCACAGGTTGCTAACGGTCG-3′ | |
21.AP-iB | 5′-GTGTTTGGCGAGATGGAGTTC-3′ | |
trpS | 24.WSA | 5′-CATCCGGCATGAACAAAGCGCAAT-3′ |
24.WSB | 5′-TTCTGCCCGCATTAGGGCTTCCGC-3′ | |
trpT | 24.WTA | 5′-GGGTCGCGGGTTCGAGTCCCGTCC-3′ |
24.WTB | 5′-CTTCGGAGAGGGTTATTTCAGATA-3′ | |
tnaA | 24.TNA | 5′-GATGGTGCTTGCATATATATCTGG-3′ |
24.TNB | 5′-GTTATTGAGGATGTAGGGTAAGAT-3′ | |
24.TN-iA | 5′-GATATGCGGCAACAGTTTACCGGC-3′ | |
24.TN-iB | 5′-GTGGCGCAGAGCAAATCTATATTC-3′ | |
ssb | 24.SBA | 5′-GTTTCCCGGATCCGAGGTCACAAC-3′ |
25.SBB | 5′-CTGGCAGATGCTTTAAGGATCCACC-3′ | |
gapA | 24.GAA | 5′-TTGACGCTGCGTAAGGTTTTTGTA-3′ |
24.GAB | 5′-AGCGACCGAAGTCGCTCTTTTTAG-3′ | |
ColE1 | 25.OCA | 5′-TAATGGTTTCTTAGACGTCAGGTGG-3′ |
24.OCB | 5′-GTAGTTCGCCAGTTAATAGTTTGC-3′ | |
bla | 26.50 | 5′-TTCTTAGACGTCAGGTGGCACTT-3′ |
27.19 | 5′-TTAAGGGATTTTGGTCATGAGATT-3′ | |
24.JB1 | 5′-AACTTTATCGGCCTCCATCCAGTC-3′ | |
trpR | 20.WRA | 5′-CCCCCGCTAACAATGGCGAC-3′ |
19.WRB | 5′-CGCCTGATGCGACGCTGCC-3′ | |
tyrR | 20.YRA | 5′-ATCTTTACGCCGAAGTGCCC-3′ |
21.YRB | 5′-CGCCTGATGCGACGCTGCC-3′ | |
23.YR-iA | 5′-TGTCGATATGAAAAGCAAAGTGG-3′ | |
26.YR-iB | 5′-GTCAACACGTTCAGACGATAATAGAG-3′ | |
mtr | 36.MTA | 5′-GGCAACGCTGTCCTACATAGACCTGATA AGCGAAGC-3′ |
38.MTB | 5′-TCGGGGCTTTTTTTCTGTCTTTTGTACTC GTGTACTGG-3′ | |
20.MT-iA | 5′-CCAGACAGAACGGCAGGGTC-3′ | |
27.MT-iB | 5′-GGCGAAAGTCATTACCTTCTTCCTCAC-3′ |
Primers amplify genes as well as ∼100 bp of 5′ and 3′ flanking sequence.
The first two primers listed for each gene were used to amplify the gene and sequence and the 5′ and 3′ ends. Any additional primers were used to sequence internal regions of the gene.