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. 2022 Sep 29;14(1):2126272. doi: 10.1080/19490976.2022.2126272

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Periodontitis-related salivary microbiota impairs intestinal barrier in PAP mice. (a-c) Representative images of ZO-1 and occludin immunostaining (a) and quantification of area fractions in the colon of PAP mice (b-c) (n = 3). Scale bar = 50 µm. (d) Relative mRNA expressions of ZO-1 and occludin (n = 6). (e) Concentrations of fecal albumin detected by ELISA (n = 6). (f-g) Alcian blue and periodic acid-Schiff (AB-PAS) staining showing the distribution of goblet cells in colon (f) and the goblet cells per crypts that normalized to the Sham group (g) (n = 3). Scale bar = 50 µm. (h) The ELISA test indicating levels of TNF-α and IL-1β in plasma of mice (n = 6). Data were presented as the mean ± SD in the different experimental groups, *p < .05, **p < .01, ***p < .001. H, healthy; P, periodontitis.