Figure 2.

OXPHOS deficiency selectively sensitizes quiescent cells to oxidative stress. (A) OXPHOSdeficiency in quiescent cells leads to increased cell death upon oxidative damage. (B) Representative images of cell death and cell proliferation analyzed by TUNEL and EdU staining, respectively, in the liver of vCTRL and PEITC-treated mice. Scale bar: 500 µm. (C) Quantification of TUNEL positivity (ratio between PEITC and vCTRL) in EdU⁺ (proliferating, PCs) and EdU⁻ (quiescent, QCs) cells (mean ± S.E.M., n = 4 mice per condition, *p < 0.05, **p < 0.01, one-way ANOVA with Sidak’s multiple comparisons test). (D and E) Cell death in EA.hy926 PCs and QCs with (ρ⁰) or without OXPHOS deficiency and treated with (D) PEITC and (E) H₂O₂ for 22 h evaluated by ANXA5-FITC and PI using flow cytometry (mean ± S.E.M., n ≥ 4, ^n.s.p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 versus parental cells, two-way ANOVA with Tukey’s multiple comparisons test).