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Time-dependent monophosphate release profile of TPP-coated PLOA-decorated NEs (PLOA/TPP NEs) incubated with 2 U (▲), 1 U (●), 0.5 U (■), 0.3 U (▼) of isolated ALP. The control experiment was the one without the addition of enzyme (◯). Data are expressed as means ± SD, n ≥ 3. The significant difference is indicated as ***p < 0.001.
