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. 2022 Mar 1;18(10):2333–2349. doi: 10.1080/15548627.2022.2029671

Figure 1.

Figure 1.

TFEB interacts with the FACT complex under stress conditions. (A) Immunoblot analysis of immunoprecipitated TFEB-Flag from HeLa cells incubated with DMSO (Control), EBSS for 4 h, Torin-1 (250 nM) for 2 h or NaAsO2 (250 μM) for 2 h. (B) Immunoblot analysis of immunoprecipitated TFEB-Flag from ARPE-19 cells infected with either Ad-Null or Ad-TFEB-S211A and incubated with DMSO (Control), EBSS for 4 h, Torin-1 (250 nM) for 2 h or NaAsO2 (250 μM) for 2 h. (C) Immunoblot analysis of immunoprecipitated endogenous SSRP1 from HeLa WT cells treated with DMSO (Control) or Torin-1 (250 nM) for 2 h. (D) Immunoblot analysis of proteins from the nuclear fraction of HeLa-TFEB-Flag cells treated with DMSO (Control), Torin-1 (250 nM) for 2 h or NaAsO2 (250 μM) for 2 h. Immunoblots are representative of at least three independent experiments.