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. 2022 Mar 1;18(10):2333–2349. doi: 10.1080/15548627.2022.2029671

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 2. — The interaction between TFEB and the FACT complex occurs in the nucleus. (A) Immunoblot analysis of TFEB-Flag immunoprecipitation from ARPE-19cells infected with Ad-Null, Ad-TFEB-WT-Flag or Ad-TFEB-S211A-Flag and treated with DMSO (Control) or Torin-1 (250 nM) for 2 h. (B) Immunoblot analysis of TFEB-Flag immunoprecipitation from ARPE-19 cells expressing TFEB-WT or the indicated TFEB mutants upon treatment with NaAsO₂ (250 μM) for 2 h. (C) Immunofluorescence confocal microscopy analysis of ARPE-19 cells showing the intracellular distribution of recombinant TFEB-WT or the indicated TFEB mutants in response to treatment with NaAsO₂ (250 μM) for 2 h. Scale bars: 10 μm.