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. 2022 Jun 23;31(8):1276–1280. doi: 10.1111/exd.14626

TABLE 1.

List of primary and secondary antibodies used

Target Type Host Concentration Supplier Accession number
Acetylated α‐tubulin (clone 6‐11B‐1) Primary, monoclonal Mouse IF: 1/1000 Sigma‐Aldrich 6793
Keratin 10 (clone DE‐K10) Primary, monoclonal Mouse WB: 1/500 NeoMarkers, Thermo Scientific MS‐611‐P0
Rootletin (CROCC) Primary, polyclonal Rabbit IF: 1/200 NovusBio NBP1‐80820
GFP (clone 3E6) Primary, monoclonal Mouse IF: 1/250 Invitrogen A‐11120
α‐tubulin (clone DM1A) Primary, monoclonal Mouse WB: 1/5000 Sigma‐Aldrich T9026
ECL™ Anti‐mouse IgG, HRP‐conjugated Secondary, polyclonal Sheep WB: 1/5000 GE Healthcare UK Limited NA931V
AlexaFluor‐488 conjugated anti‐mouse Secondary, polyclonal Chicken IF: 1/500 Invitrogen A21200
AlexaFluor‐488 conjugated anti‐rabbit Secondary, polyclonal Donkey IF: 1/500 Invitrogen A10040
AlexaFluor‐546 conjugated anti‐mouse secondary Secondary, polyclonal Goat IF: 1/500 Invitrogen A11003

Abbreviations: IF, immunofluorescence; WB, Western blot.