TABLE 1.
Target | Type | Host | Concentration | Supplier | Accession number |
---|---|---|---|---|---|
Acetylated α‐tubulin (clone 6‐11B‐1) | Primary, monoclonal | Mouse | IF: 1/1000 | Sigma‐Aldrich | 6793 |
Keratin 10 (clone DE‐K10) | Primary, monoclonal | Mouse | WB: 1/500 | NeoMarkers, Thermo Scientific | MS‐611‐P0 |
Rootletin (CROCC) | Primary, polyclonal | Rabbit | IF: 1/200 | NovusBio | NBP1‐80820 |
GFP (clone 3E6) | Primary, monoclonal | Mouse | IF: 1/250 | Invitrogen | A‐11120 |
α‐tubulin (clone DM1A) | Primary, monoclonal | Mouse | WB: 1/5000 | Sigma‐Aldrich | T9026 |
ECL™ Anti‐mouse IgG, HRP‐conjugated | Secondary, polyclonal | Sheep | WB: 1/5000 | GE Healthcare UK Limited | NA931V |
AlexaFluor‐488 conjugated anti‐mouse | Secondary, polyclonal | Chicken | IF: 1/500 | Invitrogen | A21200 |
AlexaFluor‐488 conjugated anti‐rabbit | Secondary, polyclonal | Donkey | IF: 1/500 | Invitrogen | A10040 |
AlexaFluor‐546 conjugated anti‐mouse secondary | Secondary, polyclonal | Goat | IF: 1/500 | Invitrogen | A11003 |
Abbreviations: IF, immunofluorescence; WB, Western blot.