Figure 4.

DPP4 is required for proliferation and self-renewal of human ASC. shRNA-mediated DPP4 KD in human ASC was generated and confirmed on (a) protein level and (b) gene expression level (sh1 = anti-DPP4 shRNA1, sh2 = anti-DPP4 shRNA2, sh scr = scrambled control shRNA). (c–e) In DPP4 KD ASC, (c) growth curve was assessed as a number of events recorded in 90 s with FACS, (d) BrdU incorporation assay and (e) colony formation assay was performed as a measure of proliferative and self-renewal capacity. (a, b) representative results from three donors are shown. (c–e) n = 3 donors, mean ± SEM is shown. Gene expression was normalized to β-actin, and protein expression was normalized to total protein via Ponceau S staining.