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. 2022 Oct 3;11(1):601–615. doi: 10.1080/21623945.2022.2129060

Figure 5.

Figure 5.

DPP4 negatively regulates adipogenic differentiation of human ASC. (a–f) In DPP4 KD ASC, adipogenic differentiation was assessed by the analysis of gene expression of (a) PPARγ2, (b) FABP4 and (c) Perilipin as indicated. Moreover, protein levels of (d) PPARγ2 and (e) FABP4 were measured at day 9, and (f) triglyceride accumulation on day 14 post-induction of adipogenesis. (g–n) Analysis of protein level of adipogenic marker proteins in the course of adipogenesis in DPP4 overexpressing (OE) ASC. (g, h) DPP4 overexpression (OE) in ASC confirmed on (g) protein level and (h) gene expression level. (i–n) In DPP4 OE ASC, adipogenic differentiation was assessed by the analysis of gene expression of (i) PPARγ2, (j) FABP4 and (k) Perilipin as indicated. Moreover, protein levels of (l) PPARγ2 and (m) FABP4 were measured at day 9 and (n) triglyceride accumulation on day 14 post-induction of adipogenesis. Representative results from three donors are shown, mean ± SEM is shown, gene expression was normalized to β-actin and protein expression was normalized to total protein via Ponceau S staining.