Figure 5.

Differential transcriptional profiles between naïve CD8⁺ T cells from wild‐type (WT) and Satb1^m1Anu/m1Anu mice. RNA sequencing was performed on either naïve (CD44^loCD62L^hi) CD8⁺ T cells from uninfected WT or Satb1^m1Anu/m1Anu mice, or on purified D^bNP₃₆₆/D^bPA₂₂₄ influenza A virus–specific CD8⁺ T cells 10 days after primary infection with A/HKx31. (a) Multidimensional scaling (MDS), (b) hierarchical clustering based on Z‐score and volcano plots of (c) naïve or (d) effector transcriptional profiles with highlighted genes are shown. RNA‐seq libraries were generated in triplicate from pooled samples of three to five mice per replicate. SATB1, special AT‐binding protein 1. [Colour figure can be viewed at wileyonlinelibrary.com]