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. Author manuscript; available in PMC: 2022 Oct 7.
Published in final edited form as: Anal Biochem. 2022 May 10;650:114712. doi: 10.1016/j.ab.2022.114712

Fig. 3.

Fig. 3.

A) Detection of SYBR Green fluorescence after each of three steps: anneal (51 °C), extend (72 °C), and cool (48 °C). B) SYBR Green qPCR profiles monitored for the same reaction after these three steps: extend (blue), cool (green), and anneal (red). C) Interpretation of qPCR product complexes detected at early and late cycles for DNA libraries containing a central N40 random region.