Figure 3.

Significant enrichment of microbial MetaCyc pathways based on environments (urban vs. rural) (a–c) and allergies (d–f) in children’s gut microbiota. Associations with MetaCyc pathways are analyzed by MaAsLin2 and adjusted for age, gender, and BMI. (a, d) Positive and negative correlations of coefficients by MaAsLin2 as indicated by red and blue color, respectively. (b, e) P-values adjusted for multiple comparisons, and Benjamini–Hochberg adjusted p-values with an FDR < 0.25 shown. (c, f) Relative pathway abundance log-transformed and shown for urban vs. rural and case vs. control. Refer to Table S4 for the MaAsLin2 associations.