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. 2021 Nov 25;17(10):1219–1233. doi: 10.1080/15592294.2021.2003043

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Gene expression analysis during SGBS cellular differentiation.

Legend: Triplicate qRT-PCR results from markers of cell differentiation genes: panels (a) CHI3L2-220, panel (b) CHI3L2-217, panel (c) CEBPA, panel (d) LBP, panel (e) LEP, and panel (f) PPARG; the triplicate experiments (exp) are labelled 01 (blue), 02 (red) and 03 (green) and the fourth curve in black (downward triangle) is the mean. The CHI3L2 qRT-PCR assays targeted exons 6–7 (panel a:220-CHI3L2) and exons 3–4 (panel b:217-CHI3L2) using Thermofisher TaqMan® gene expression assays ID Hs00970220_m1 and Hs00970217_m1 respectively.