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Summary of cell culture B12 and folate conditions in discovery experiment and validation experiment.
| CHUB-S7 | Discovery experiment |
Validation experiment |
||
|---|---|---|---|---|
| Conditions | B12 (nM) | Folate (μM) | B12 (nM) | Folate (μM) |
| No B12 | 0 | 6 | 0 | 6 |
| Low B12 | 0.15 | 6 | 0.15 | 6 |
| Control (‘normal B12') | 500 | 6 | 500 | 6 |
| Control + methylation inhibitor | 500 + 5-Aza-dC | 6 | Not done | Not done |
Legend: 5-Aza-dC is the methylation inhibitor 5-aza-2ʹ-deoxycytidine
