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Validation of differentially expressed known and novel miRNA and their respective target gene by qRT-PCR. U6 RNA gene was used as reference gene for miRNA and EF-1 alpha for target gene. Data are mean ± SD from three biological replicates determined by Student t test. a miRNA and their respective target under AtECS1 Control vs Col-0 Control (AC vs CC). b miRNA and their respective target under AtECS1 Infected vs Col-0 Infected (AI vs CI).
