Figure 7.

A typical ASMD simulation is divided into 20 stages, as shown, and sampled from 100 trajectories per stage at a stretching velocity, v_s , of 10 Å/ns. for the α‐helical peptide 1PEF. The solid black lines represent the work for each trajectory, W(${\zeta }_t^{\left(i\right)}$ ), whereas the thick red curve represents the overall PMF, $\bar{W}$ , from Eq. 2.