FIGURE 1.

Schematic representation depicting the stages of forming reconstructed human pigmented skin and epidermis. Reconstructed human pigmented skin (RHPS): On day 0, fibroblasts (neonatal human dermal fibroblasts, HDFn) are seeded onto the scaffold to allow the formation of the dermis. On day 7, the formation of the epidermis begins with the addition of melanocytes (human epidermal melanocytes from darkly pigmented skin, HEM‐DP), followed by addition of keratinocytes (neonatal human epidermal keratinocytes, HEKn) on the following day. Three days after, apical media is removed to achieve the air‐liquid interface and on day 24, the model is fully formed. Reconstructed human pigmented epidermis (RHPE): This model is formed on a collagen‐coated membrane to support melanocytes and keratinocytes. On day 1, the melanocytes are seeded, followed by keratinocytes, on the following day. One day after, the air‐liquid interface is established and on day 15, the epidermis is completed