TABLE 2.
Analyses of different body parts of Culicoides nubeculosus for the presence of lumpy skin disease virus DNA by quantitative polymerase chain reaction (qPCR) a or viable virus by cell culture inoculation at different time points after exposure to infectious blood meals.
| (a) First experiment | |||
|---|---|---|---|
| Day post‐infectious blood‐meal | qPCR (head) | qPCR (body b ) | CPE+ in cell culture (body) |
| No. positive/no. tested | |||
| 0 | 1/10 | 5/5 | 4/5 |
| 1 | 0/10 | 5/5 | 5/5 |
| 2 | 1/10 | 4/5 | 1/4 |
| 4 | 2/10 | 0/5 | nd |
| 5 | 1/10 | 0/5 | nd |
| 7 | 3/10 | 3/5 | 0/3 |
| 10 | 3/10 | 2/5 | 2/2 |
| (b) Second experiment | ||||||
|---|---|---|---|---|---|---|
| Day post‐infectious blood‐feeding | qPCR (head) | qPCR (abdomen) | qPCR (wings) | CPE in cell culture (head) | CPE in cell culture (abdomen) | CPE in cell culture (wings) |
| No. positive/no. tested | ||||||
| 1 | 3/10 | 2/5 | nd | 0/3 | 0/2 | nd |
| 10 | 1/38 | 1/20 | 1/16 | 1/1 | 1/1 | 1/1 |
Abbreviations: CPE, cytopathic effect (all qPCR‐positive specimens examined); nd, not done.
a
Positive: Cq ≤ 34.6.
b
Body: thorax and abdomen. Bodies (a) or abdomens only (b) investigated from all specimens qPCR‐positive in the head samples, complemented with randomly chosen samples.