TABLE 3.

Analyses of different body parts of field‐collected Culicoides spp. for the presence of lumpy skin disease virus DNA by quantitative polymerase chain reaction (qPCR) ^a or viable virus by cell culture inoculation at different time points after exposure to infectious blood meals.

Day post infectious blood meal	qPCR (head)
	No positive/no tested	qPCR (body b )	CPE in cell culture (body)
0	0/10	3/5	3/3
1	3/24	0/5	nd
3	0/15	0/5	nd
5	0/15	0/5	nd
7	0/20	0/5	nd
10	0/15	0/5	nd

Abbreviations: CPE, cytopathic effect (all qPCR‐positive bodies examined); nd, not done.

^a Positive: Cq ≤ 34.6.

^b Body: thorax and abdomen (bodies investigated from all specimens qPCR‐positive in the head samples, complemented with randomly chosen samples).