FIG. 2.

Segments transferred to pGETS103. (A) SalI digests of plasmid from the BEST2012 transformant. Two antibiotic resistance genes (cat and erm) originating from genomic pBR322 (Fig. 1B) were transferred to all plasmids. (B) BstEII digests of the lambda DNA insert in the plasmid are compared with intact lambda digested by BstEII. Plasmids from 20 transformants each of BEST2037 (18.2-kb segment), BEST8133 (29.2-kb segment), BEST2045 (42.5-kb segment), and BEST8046 (50.1-kb segment) showed faithful recombinational transfer, except for clones denoted by the downward arrow as described in the text.