FIGURE 22.

Low (a–f) and higher (insets a–f) magnification photomicrographs of coronal sections through the major Island of Calleja (ICjM) of the tree pangolin brain stained for Nissl (a), neuronal nuclear marker (NeuN; b), choline acetyltransferase (ChAT; c), calbindin (d), calretinin (e), and vesicular glutamate transporter 2 (vGlut2; f). Note the high density of small neurons forming the ICjM (a and b), some of which are calretinin immunopositive (e). A high density of ChAT immunopositive boutons (c) and lower densities of calbindin (d) and vGlut2 (f) boutons. A high density of vGlut2 boutons was observed in the adjacent nucleus accumbens (Acb; f). In all photomicrographs, medial is to the left and dorsal to the top. Scale bar in (f) = 500 μm and applies to all. Scale bar in inset (f) = 20 μm and applies to all insets. See list for abbreviations