Table 2.

ERO1α primer sequences for nMS‐PCR

Gene	Species	Primer Sequence (5′→3′)
Outer primer	Mouse	Forward: 5′‐GTTTTGGGAATTGGAAGGATT‐3′
		Reverse: 5′‐AAAAAAAATAAAACAAATTATTAAACTTAC‐3′
Methylation primer	Mouse	Forward: 5′‐ACTAACCTACAATAATACGATCGCA‐3′
		Reverse: 5′‐AGTTTGGGTAATATGG TGAAATTTC‐3′
Unmethylation primer	Mouse	Forward: 5′‐ACTAACCTACAATAAT ACAATCACA‐3′
		Reverse: 5′‐AGTTTGGGTAATAT GGTGAAATTTC‐3′

Abbreviations: ERO1α, Endoplasmic reticulum oxidoreductase 1α; nMS‐PCR, nested methylation‐specific polymerase chain reaction.