FIG. 3.

Phenotype of transformants of P. chrysogenum Wisconsin 54–1255 expressing pahA and phacA gene constructions. The analysis was carried out with the untransformed strain Wisconsin 54–1255 (diamonds), transformant T-06 carrying an extra copy of endogenous pahA (squares), and transformant T-16 carrying phacA from A. nidulans (triangles). (A and B) Penicillin G production and PA consumption in penicillin fermentation conditions. Flask fermentations were carried out in standard conditions measuring penicillin G production and PA consumption by HPLC at the time points indicated. Transformant T-16, carrying phacA, showed a strong reduction in penicillin production and enhanced PA consumption. (C) PA oxidation test in liquid minimal medium. Mycelium of each strain was grown on glucose-supplemented medium for 24 h before transfer to minimal medium with PA as the sole carbon source. Flasks were shaken at 25°C, and samples were taken at the time points indicated to quantify the remaining PA by HPLC. Again, only T-16 showed capacity to consume PA. Vertical bars indicate standard deviations.