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. 2022 Jun 14;30(4):421–435. doi: 10.1111/wrr.13033

FIGURE 4.

FIGURE 4

(A) Histological and immunohistological evaluation for identifying the angiogenic activity of gelatin/hyaluronic acid hydrogels, in their pristine and loaded with adipose‐derived stem cells (ASCs) and VEGF forms, on Day 14 by using the chick embryo chorioallantoic membrane (CAM) assay. Arrows and arrow heads display large and small blood vessels, respectively. (B) The chart exhibits the quantified fluorescent α‐SMA positive blood vessel area (**p < 0.01, ***p < 0.005). (C) Images taken from (upper row) haematoxylin and eosin (H&E) stained samples indicating a lack of inflammation and slight perivascular inflammatory cell infiltration on the CAM tissue adjacent to the un‐loaded, ASC‐, and VEGF‐loaded hydrogels; (lower row), fluorescently stained samples indicating more α‐SMA stained blood vessels on the CAM underneath ASCs loaded and VEGF‐loaded hydrogels (Scale bars: 100 μm). Source : Reproduced with permission from Ref. 115