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. 2022 Jun 29;37(8):1545–1561. doi: 10.1002/jbmr.4616

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© 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 2 — Single‐cell RNAseq of periosteal cells at steady state. (A) Experimental design of scRNAseq of PCs at steady state. PCs were isolated from uninjured tibia of Prx1 ^Cre ;Rosa ^mTmG mice by explant culture without expansion and subjected to scRNAseq analyses at P0. (B) Left: clusterization of PCs. Right: Feature plot of eGFP expression. (C) Expression of markers used to define SP, Macro, and Oc clusters. (D) Representation of SP, Macro, and OC marker expression. Macro = macrophage; Oc = osteoclast; P0 = passage 0; PC = periosteal cell; SP = stem/progenitor.